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A selenium-dependent glutathione peroxidase cDNA was obtained from haemocyte of green mud crab Scyllaparamamosain (SpGPx) by homology polymerase chain reaction (PCR) technique and rapid amplification of cDNA ends (RACE) methods.The 1135 bp cDNA contains a 9 bp 5-untranslated region (UTR), an open reading frame (ORF) of 564 bp encoded a deduced amino acid sequence of 187 amino acids (aa), and a 562 bp 3-untranslated region with a 100 bp conserved eukaryotic selenocysteine insertion sequence (SECIS).