【摘 要】
：
In order to increase the yield of CLA, we studied the biosynthesis of CLA by Linoleate isomerase under supercritical CO2 conditions.Five factors including p
【机 构】
：
Institute of Agro-Food Science and Technology, Chinese Academy of Agricultural Sciences, Key Laborat
【出 处】
：
2011 Beijing Internation Conference on Non-thermal Processin
论文部分内容阅读
In order to increase the yield of CLA, we studied the biosynthesis of CLA by Linoleate isomerase under supercritical CO2 conditions.Five factors including pH, temperature, LA concentration, cell concentration and pressure were taken into consideration, and then the conversion mechanism was studied by using of GC, SDS-PAGE and SEM.The results showed that the optimal conditions were as follows: pH 7, temperature 37℃, LA concentration 1mg/mL, cell concentration 25%, and pressure 15MPa.The CLA yield was 600μg/mL and conversion ratio was 60% under the above conditions.
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