论文部分内容阅读
Modern separation and detection instrumentations have greatly promoted analytical performance, through the whole workflow of which, sample preparation is becoming the main bottleneck of analytical throughput.For analyses of complex biosamples, most of the time target analytes are shadowed in background interferents, such as salts, small molecules and other additives.Removal of interferents and meanwhile enrichment of fractions of interest are prerequisites for successful analysis at downstream.Taking into account the limit amount of available biosamples that normally encountered, a microscale sample preparation device is in quest for bioanalysis.Based on a newly introduced single particle fritting technology, very short capillary columns, microbullets, can be fabricated with a length of 0.5 to 1 cm.As a microscale solid phase extraction column, such microbullet can effect as a trapping, desalting and preconcentration device.Liquid samples smaller than 10 microlitre can be easily handled by such device.With protein digests as samples, the versatile functions of microbullet were proved, initial results clearly demonstrated potential applications of such micro device in bioanalytical area where sample availability is normally limited.