【摘 要】
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Objectives: To investigate the miRNA profile in the corpus cavernosum (CC) of aging rats with ED, and to analyze the target genes and signaling pathways regulated by the dysregulated miRNAs.Methods: A
【机 构】
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Department of Urology,Affiliated Drum Tower Hospital,Nanjing University,School of Medicine,Nanjing,2
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Objectives: To investigate the miRNA profile in the corpus cavernosum (CC) of aging rats with ED, and to analyze the target genes and signaling pathways regulated by the dysregulated miRNAs.Methods: According the apomorphine test, the experimental animals were divided into three groups: aging rats with ED (group AE), aging rats with normal erectile function (group AN), and young rats as normal controls (group YN).After the erectile functional test, CCs from each group were then collected for histological and molecular measurements.Results: Accompanied with significantly decreased erectile function, the content of smooth muscle and endothelium within the CC of rats with A-ED was significantly decreased compared with both AN group and YN group.miR-1, miR-20Oa, miR-203, and miR-206, were found and validated up-regulating with above 2-fold change in AE group.According to the bioinformatics analysis, the four up-expressing miRNAs could regulate eNOS/NO/PKG and PGE1/PKA pathways through regulating 13 target genes.Conclusions: Four miRNAs were found up-regulated significantly in the CC of rats with A-ED.The four miRNAs might play important roles in the development of A-ED by regulating the eNOS/ NO/PKG and PGE1/PKA pathways.
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