【摘 要】
:
甘蓝型油菜种子类黄酮和羟基苯丙烯酸衍生物的全面了解对于油菜种子的品质改良具有重要的意义。目前HPLC-PDA-ESI(-)/MSn/HRMS方法被广泛用于植物多酚物质组成的研究。本研究通过提取甘蓝型油菜种子的多酚物质,并对粗提物进行Sephadex LH-20柱层析分离、碱性水解和不同分离组分的HPLC-PDA-ESI(-)/MSn(IonTrap)和HPLC-DAD-ESI(-)/HRMS(Q-
【机 构】
:
扬州大学生物科学与技术学院,江苏扬州,225009
【出 处】
:
江苏省植物生理学会第九届会员代表大会暨2014年学术年会
论文部分内容阅读
甘蓝型油菜种子类黄酮和羟基苯丙烯酸衍生物的全面了解对于油菜种子的品质改良具有重要的意义。目前HPLC-PDA-ESI(-)/MSn/HRMS方法被广泛用于植物多酚物质组成的研究。本研究通过提取甘蓝型油菜种子的多酚物质,并对粗提物进行Sephadex LH-20柱层析分离、碱性水解和不同分离组分的HPLC-PDA-ESI(-)/MSn(IonTrap)和HPLC-DAD-ESI(-)/HRMS(Q-TOF)分析,成功发现了91种类黄酮和羟基苯丙烯酸衍生物,其中包括山奈酚衍生物39种、异鼠李素衍生物11种、槲皮素衍生物5种、黄烷醇及其低聚物6种、羟基苯丙烯酸衍生物30种。成功鉴定了上述化合物中的78种次生代谢物,其中55种为甘蓝型油菜首次报道,24种为芸薹属植物首次报道。
其他文献
Understanding the genetic and molecular basis of resistance to stem rust is important for devising effective approaches to control this devastating biotrophic pathogen.
Yellow or stripe rust (YR),caused by the fungus Puccinia striiformis f.sp.tritici (Pst) is a major wheat disease in the United Kingdom and can cause extensive yield loss.
The wheat breeding program at CIMMYT has a long history in pioneering durable stripe rust resistance breeding strategies that focus on incorporating multiple slow rusting,minor loci that confer partia
International Winter Wheat Improvement Program (www.iwwip.org) is a project established jointly in 1986 by the Ministry of Food,Agriculture and Animal Husbandry of Turkey,CIMMYT,and ICARDA.
Wheat cultivar Xingzi 9104 (XZ) possesses adult plant resistance (APR)to stripe rust caused by Puccinia striiformis f.sp.tritici (Pst).In this study,histological and cytological experiments were condu
Global wheat production is currently threatened by Ug99 lineage races of stem rust.Although most of the stem rust resistance genes (Sr) derived from alien species can effectively control Ug99 virulenc
Stripe rust,caused by Puccinia striifomis f.sp.tritici (Pst),is one of the most destructive diseases of wheat in China,generally causing 5%-10% yield losses.
Tenuazonic acid (TeA),a phytotoxin produced by the fungus Alternaria alternata isolated from diseased croftonweed (Ageratina adenophora),exhibits a strong inhibition in photosystem Ⅱ (PSⅡ) activity.In
cdns是一种环化酶,由一个复杂的基因家族所编码,它催化(E,E)-法呢基焦磷酸(FPP)环化形成(+)-δ-杜松烯,杜松烯是一类重要的植保素.本研究以pCAMBIA1301为载体,利用玉米泛素(Ubiquitin)启动子构建Oscdns过表达载体,并通过农杆菌介导法将Oscdns基因转化到台北309 (Tp309)中,获得再生苗15株,对再生苗进行GUS染色、PCR鉴定,超表达植株T-8、T-1
In plants,basic leucine zipper (bZIP) transcription factors play pivotal roles in the abiotic stress response.In this study,we cloned GmbZIP110,a bZIP transcription factor gene from soybean (Glycine m