【摘 要】
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Sodium and divalent cations are involved in cohesion of the bacterial biofilms formation.However, the effects of sodium, magnesium, and calcium ions on biofilm formed by Cronobacter sakazakii strains
【机 构】
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School of Biotechnology and Food Engineering,Hefei University of Technology,Hefei 230009,China
【出 处】
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第五届全国微生物资源学术暨国家微生物资源平台运作服务研讨会
论文部分内容阅读
Sodium and divalent cations are involved in cohesion of the bacterial biofilms formation.However, the effects of sodium, magnesium, and calcium ions on biofilm formed by Cronobacter sakazakii strains are not known.The biofilm-forming ability of 23 Cronobacter sakazakii strains was assessed at different NaCl (0.00%, 0.25%, 0.50%, 1.50%, 2.00%, w/v), MgCl2 (0.00%, 0.25%, 0.50%, 1.00%, 1.50%, w/v) and CaC12 (0.00%, 0.25%, 0.50%, 1.00%, 1.50%, w/v).Biofilm formed by Cronobacter sakazakii strains was evaluated in tryptone soy broth in 96-well plates using crystal violet staining and its quantification was counted using the optical density measurements (OD570nm) at 28℃ for 24h of incubation.
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