Some natural polysaccharides possess important biological activities such as immunity adjustment, anti-virus, and anti-tumor activity and so on.A significant problem in study of the polysaccharide structure is analysis of monosaccharides compositions.In this paper, a simple and sensitive pre-column derivatization method for simultaneous determination of eight neutral,two acidic and two basic monosaccharides labeled with 1-phenyl-3-methyl-5-pyrazolone(PMP) has been established by high performance liquid chromatography.The effects of volume proportions of acetonitrile and pH values of mobile phase(0.1mol/L phosphate buffer-acetonitrile) on retention and separation of PMP derivatives of ten monosaccharides were investigated with Eclipse XPB-C1s Column (4.6 mm× 250 mm) screened out suitable to separate the monosaccharide derivatives from six brands of reversed-phase columns.