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目的 :探讨不同材料人工关节磨损颗粒对界面细胞生长特性的影响。方法 :以体外培养正常人髋关节滑膜细胞系为模型 ,分别加入 0 5和 1 5mg/ml(W /V)的Ti合金 (Ti 6A1 4V)、CoCr合金和UHMWPE颗粒 ,观察滑膜细胞的生长曲线和倍增时间及其对颗粒的毒性反应。结果 :与空白对照相比 ,UHMWPE颗粒对细胞生长有促进作用 ,倍增时间 2 4 9h(空白对照为 2 5 3h) ;Ti合金颗粒早期抑制细胞生长 ,第 4天开始细胞数量持续增长 ,倍增时间为 32 7h ;CoCr合金颗粒表现为细胞毒性作用 ,至第 8天细胞全部死亡 ,其毒性作用与颗粒浓度有关。结论 :不同的人工关节材料颗粒对体外培养滑膜细胞的作用不同 ,其表现与之在松动人工关节界膜组织中的反应相似。
OBJECTIVE: To investigate the effect of different materials of artificial joint wear particles on the growth characteristics of interfacial cells. Methods: Human normal synovial cell line was cultured in vitro as a model, Ti alloy (Ti 6Al 4V), CoCr alloy and UHMWPE particles of 0 5 and 1 5 mg / ml (W / V) Growth curves and doubling times and their toxic effects on the particles. Results: Compared with the blank control group, UHMWPE particles could promote cell growth with a doubling time of 249 hours (control: 253 hours). The Ti alloy particles inhibited cell growth at the early stage and continued to grow on the fourth day. The doubling time Was 32 7h; CoCr alloy particles showed cytotoxicity, and all the cells died on the 8th day. The toxic effect was related to the particle concentration. CONCLUSION: Different artificial joint material particles have different effects on synoviocytes cultured in vitro, and their performance is similar to that in synovial articular tissue.