The importance of polymeric biomaterials has been recognized in biomedical research for over four decades.The response of biomaterials in a biological environment is characteristically associated with their surface properties.The modification of polymeric biomaterials by various surface treatments by various surface treatments has become an active topic in surface engineering.A number of research group have focus on the preparation of surfaces with a gradually varying chemical composition along one dimension.Such a "gradient surfaces" is of particular interest for basic and applied studies of the interactions between biological species and surfaces as the dependence of a selected property,such as wettability,on composition,can be examined in a single experiment on one surface.In this presentation,we,describe the preparation and characterization of gradient polymer surfaces,presenting overview of the progress on over two decades in my laboratory.The introduction and characterization of biofunctional groups on gradient surface surfaces will be presented.Finally,we concentrate on the interaction of these surfaces with biological species,such as proteins and cells,important in the understanding of the basic science for biomedical applications.1.2 An appropriate cellular response to implanted surfaces is essential for tissue regeneration and integration.