【摘 要】
:
Influenza A virus (IAV) infects the epithelial cells that line the surface of the respiratory tract, thereby inducing prominent necrotizing bronchitis and variable interstitial pneumonia.The severity
【机 构】
:
Key lab of Experimental Animal, Zhejiang Academy of Medical Sciences, Hangzhou 310013, China
【出 处】
:
华东地区第13届实验动物科学学术交流会
论文部分内容阅读
Influenza A virus (IAV) infects the epithelial cells that line the surface of the respiratory tract, thereby inducing prominent necrotizing bronchitis and variable interstitial pneumonia.The severity of IAV infection is closely related to the dysfunction of apoptosis regulation.MicroRNA has distinct expression profiles in innate immunity, inflammation, and viral infection.However, the function of microRNA in IAV infection remains unknown.In this study, the function of microRNA in the development of IAV infection was investigated to identify potential drug targets.MicroRNA-155 (miR-155) expression in the lung tissues of IAV-infected mice was found to be significantly upregulated and peaked 5 d post-IAV challenge.MiR-155 overexpression in A549 cells significantly promoted cell apoptosis by inducing S-phase cell-cycle arrest.MiR-155 overexpression also inhibited the protein expression of phosphoinositide 3-kinase catalytic subunit alpha, a direct target of miR-155 identified by bioinformatics, thereby suppressing the PI3K/Akt pathway.Moreover, repressed miR-155 effectively attenuated IAV-induced interstitial pneumonia, but failed to reduce the viral load of the lung tissue in IAV-infected mice.Therefore, regulating or inhibiting miR-155 may be of therapeutic potential in influenza pneumonia.
其他文献
目的 天然药物及其单体化合物的现代药理学评价,是现代中药研究和使中药走向世界的关键。综述近年来用于治疗肝纤维化天然药物的活性提取物及单体活性成分的研究进展。方法 基于当前肝纤维化的相关基础研究,从病因、作用机制、天然药物、抗肝纤维化活性成分等方面综合分析,对35种天然药物的有效部位萜类及其苷和甾醇、总黄酮和总皂苷类、香豆素类、生物碱类、木脂素类、氨基酸/多肽和杂环类、多糖类等进行分析,并对中草药活
Objective Understanding the natural history of chronic hepatitis C (CHC) may assist clinical decision-making and prompt medical intervention to decrease the development of complications.It has been su
目的 研究透明质酸(HA)配接激活CD44对人肝癌细胞株干性的影响.并探究丙酮酸激酶2型(PKM2)在该信号通路中起到的作用及其激活机制.方法 以人肝癌细胞株(MHCC97H,HepG2)为靶细胞.用HA处理细胞,比较HA激活CD44对肝癌细胞特性的影响.构建PKM2慢病毒敲出细胞,验证PKM2在HA-CD44信号通路中起到的作用.构建PKM2磷酸化位点突变质粒,验证PKM2在通路中发挥作用的机制
目的 评价肝穿刺活检对某些疑难肝病的诊断价值.方法 收集我院2010年1月-2014年2月临床不能确诊的疑难肝病患者行肝穿刺活检,分析其临床与病理组织学特点.结果 共有57例疑难肝病患者入选.肝穿刺取材全部成功,穿刺成功率100%.无明显疼痛、出血及血肿等严重并发症发生.患者疾病范围涉及肝脏占位性病变29例(经病理组织学证实肝细胞癌8例,占27.6%),转移性肝癌10例,占34.5%,胆管细胞癌2
Aim A novel amphipathic curcumin derivative(Curc-OEG)that inchdes two short hydrophilic ethylene glycol oligomer(OEG)chains attached to the basic curcumin structure showed improved bioavailability and
Histones are a family of basic proteins that associate with DNA in the nucleus and play key roles in regulating chromatin structure and dynamics.Emerging evidence indicates that histones can function
目的:筛选培育眼部突变表型小鼠,为人类相关疾病的研究提供材料.方法:采用乙烷基亚硝基脲(ENU)诱变处理G0代小鼠,繁育获得G1代,筛选眼部突变表型个体,进行遗传力试验,临床诊断及病理学观察.结果:本实验繁殖G1代小鼠2782只,经筛查获得眼部突变表型小鼠65只,可稳定遗传小鼠3例,分别表现为:角膜混浊、小眼球和虹膜异常等特征.角膜混浊者其角膜症状严重程度差异较大,角膜病变部位明显增厚,部分伴有新
Hypodermin A (HA), a serine protease secreted by first-instar larvae of Hypoderma lineatum (Diptera: Oestridae) is associated with inflammatory and the specific immune responses in cattle hosts.In the
目的:探讨经前期综合征(PMS)肝气郁证模型大鼠海马脑区μ阿片受体(MOR)分布形态、蛋白水平表达,初步揭示PMS肝气郁证的发生机理及舒郁胶囊对该病证的干预作用.方法:采用慢性束缚应激法复制PMS肝气郁证模型大鼠,分别予以调肝方药舒郁胶囊进行干预.以正常组大鼠作对照,模型组和舒郁组采用免疫荧光标记(IF)和蛋白免疫印迹(WB)对各组大鼠海马脑区进行检测.结果:与正常组大鼠相比,模型组大鼠海马脑区M