Sec24C-dependent transport of claudin-1 is required for hepatitis C virus entry

来源 :The 5th International& 14th National Symposium On MembraneBi | 被引量 : 0次 | 上传用户:fishonscreen
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
  Claudin-1 is a HCV co-receptor required for viral entry.While extensive studies have focused on claudin-1 as an anti-HCV target,little is known about how claudin-1 is regulated by host vesicular transport.Here we found that claudin-1 associated with Sec24C,a cargo sorting component of COPⅡ.Sec24C was partially colocalized with claudin-1.The carboxyl terminal YV motif in claudin-1 was required for the interaction between claudin-1 and Sec24C,tight junction localization of claudin-1,and HCV entry.Blocking COPⅡ transport by siRNA or inhibitor reduced surface level of claudin-1 and HCV entry.Moreover,inhibition of HCV entry by siRNA against claudin-1 was rescued by siRNA-resistant claudin-1 but not claudin-1-YV/AA.Our data identified claudin-1 as a cargo of COPⅡ transport.By interacting with the cargo sorting component of the COPⅡ machinery,claudin-1 is transported from ER to Golgi,eventually to tight junction.Blocking COPⅡ transport will inhibit HCV entry through reducing protein level of claudin-1 in tight junction.Our work will shed mechanistic insight on how HCV entry is regulated by COPⅡ transport.
其他文献
会议
会议
会议
会议
会议
会议
  Apical extracellular matrix (aECM) plays a central role in epithelial tube geometry regulation.In the Drosophila tracheal system,a secreted chitin deacetyla
  γ-L-glutamyl-S-[2-[[[3,4-dihydro-2,5,7,8-tetramethyl-2-(4,8,12-trimethyltridecyl)-2H-1-benzopyran-6-yl]oxy]carbonyl]-3-[[2-(1H-indol-3-yl)ethyl]amino]-3-ox
会议
会议