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Rice blast caused by Magnaporthe grisea was one of the three major diseases, which seriously affected the rice production.Alpha-momorcharin (α-MC), a kind of ribosome-inactivating proteins (RIPs) isolated from Momordica.charantia seeds, had obvious antifungal effect in vitro.In this study, the α-MC gene was constitutively expressed under the control of CaMV35S promoter in transgenic rice (Oryza sativa L.) by Agrobacteriumtumefaciens-mediated method.