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Black spot is a big threat to the storage of sweet potato,and the chitinase is a probably enzyme to defense black spot.In this study,the two copies of chitinase gene was first isolated and quantitative from twenty-four cultivars.The result of semi-quantitative(RT-PCR)and quantitative(Real-time PCR)were showed positive correlation with the physiological character about the resistance of black spot of the twenty-four cultivars.