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Introduction ColorectalCancer(CRC)isoneofthemostprevalenttypesofcancerdeathworldwide.ProtonMagneticresonance Spectroscopy(1HMRS)hasbeenshowntobeparticularlyusefulforidentifyingmetabolicprofilesofurinespecimens,andmay revealpotentialbiomarkersofCRC.ThispresentpilotstudyisaNMR-basedmetaboliteprofilingaimedtoevaluatetheabilityto characterizethemetabolic“fingerprint”ofurinesamples,forroutinescreeningofCRCinChina. Mateirials and Methods Urinesampleswerecollectedfrom64CRC(stageI/II:23;stageⅢ:19;stageIV:13;unknow:9)and40 healthycontrols.Allspectrawerepreprocessedandthenbucketedwiththeequalwidthof0.004ppm.Theregionofδ4.5~5.5 wasdiscardedtoeliminatetheimperfectwatersuppression.Eachbucketwasnormalizedtothetotalintegralofthespectrum priortoOPLS-DAusingtheSIMCA-P+14.0. Result Thestandardone-dimensionspectrumgaveanoverviewofallmetabolites.GooddiscriminationbetweenCRCandcontrol groupswasachievedbyOPLS-DAscoresplotgeneratedfrom1HNMRspectraofurineextracts,whichseparatedfromeachother clearly.Thestage-relatedandlocationrelatedCRCgroupshasshownagoodseparation.AccordingtotheVIPvalue>1and p<0.05,weselectedincreasedmetabolites,aetoaceticacid,guanidoaceticacid,cis-aconiticacid,anddecreasedmetabolites,Lasparagine,B-alanine,isocitricacid,hippuricacid,methylamine,L-cysteine. Conclusion Thispreliminaryresultshadshownthat1HNMRspectroscopycombinedwithmultivariatestatisticsisabletodetect consistentmetabolicalterationsintheurinaryprofileofCRCpatientswithhighsensitivityandspecificity.Thealteredurine metabolitespotentiallyrefertothedisruptionofthenormalbacterialecology,changesofTCAcycleandaminoacidmetabolism. ItmayrevealthemetabolitepathwayofCRCandmayextendourunderstandingofcolonicmolecularpathogenesisunderlying diseaseprocesses.