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目的:建立RP-HPLC法测定八维钙锌片中维生素D2和维生素E含量的方法。方法:采用Phenomenex-C18柱(4.6 mm×150 mm,5μm);以为甲醇流动相;紫外检测波长为265 nm;流速为1 mL.min-1。结果:维生素D2的线性范围为0.8473～4.2365μg.mL-1(r=0.9996),平均回收率为99.7%,RSD%为1.82%(n=9);维生素E的线性范围为0.1625～0.8125mg.mL-1(r=0.9996),平均回收率为100.3%,RSD%为1.93%(n=9)。结论:本方法操作简便,结果准确、可靠,可同时测定八维钙锌片中维生素D2和维生素E的含量。
Objective: To establish a method for the determination of vitamin D2 and vitamin E in eight-dimensional calcium-zinc tablets by RP-HPLC. METHODS: Phenomenex-C18 column (4.6 mm × 150 mm, 5 μm) was used as the mobile phase of methanol. The UV detection wavelength was 265 nm and the flow rate was 1 mL.min-1. Results: The linear range of vitamin D2 was 0.8473 ~ 4.2365μg.mL-1 (r = 0.9996), the average recovery was 99.7% and the RSD% was 1.82% (n = 9). The linear range of vitamin E was 0.1625-0.8125mg mL-1 (r = 0.9996). The average recovery was 100.3% and the RSD% was 1.93% (n = 9). Conclusion: The method is simple, accurate and reliable, and can simultaneously determine the content of vitamin D2 and vitamin E in eight-dimensional calcium and zinc tablets.