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Aim: The purpose of the present study was to define the effects of N-arachid0nylethanolamine (anandamide), an endogenous ligand for cannabinoid receptors CB1 and CB2 on the regulation of atrial natriuretic peptide (ANP) release.Methods: Isolated perfused rabbit atria were prepared.Immunoreactive ANP in the perfusate was measured by a specific radioimmunoassay.