【摘 要】
:
It is now realized that mammalian cells can produce more than 200 different forms of what are referred to as microRNAs.These are small 21-26 nucleotide RNA
【机 构】
:
FoxChaseCancerResearch,USA
【出 处】
:
2005 World DNA and Genome Day(2005中国(大连)国际DNA和基因组节大会)
论文部分内容阅读
It is now realized that mammalian cells can produce more than 200 different forms of what are referred to as microRNAs.These are small 21-26 nucleotide RNA species,that can function to negatively regulate the translation or stability of specific mRNA species.The present study is concerned with miR-122,a liver-specific microRNA.Some years ago Buendia and colleagues characterized a liver tumor in which a gene,which they called hcr,was translocated to c-myc,thereby profoundly activating c-myc expression.Later,Tuschl and colleagues showed that miR-122,the most abundant microRNA in the mammalian liver,was derived by RNA-processing of mRNA transcripts of this same hcr gene.We have found that liver-specific accumulation of miR-122 begins during embryonic development.At the same time,the levels of a predicted target for miR-122 action undergo a corresponding liver-specific decrease.The mRNA for this target,CAT-1,a cationic amino acid transporter,is predicted to contain at least 6 target sites for miR-122 action.We have used a reporter assay to show that three of these target sites are sufficient to render a gene susceptible to miR-122 action.Our miR-122 studies contribute to what will ultimately be many searches of the origin and function(s) of mammalian microRNA species.
其他文献
Diet can interfere with immune cell functions both in humans and domestic animals.Recently,molecular approach to nutritional studies carried out in humans o
目的:分析血常规检验过程中可能发生的误差,从而进一步提高血常规检验结果的准确性.方法:随机选择我院在2019年1月至2019年12月期间进行血常规检验的100例血液标本作为本次研
目的:研究个体化护理对胆结石手术患者心理状态及生活质量的影响.方法:本研究共纳入36例胆结石手术患者,选取时间段均为2019年1月-2020年1月,将其分为观察组(n=18)与对照组(n
Hip fracture is a major public health problem in Western countries and a rapidly growing problem in Asia and Latin America.The relative importance,and the m
新经济常态,人们的生活品质日益提高,糖尿病发病率居高不下,已经发展成为了危害人类健康的公共疾病之一.近年来,随着医学技术的日益成熟,各种新研发的口服降糖药不断涌现,增
Cellula r senescence is a general stress-response program that restrains cellular proliferation.We recently demonstratedthat telomere-initiated cellular sen
The rapid increase in genomic information requires new techniques to infer protein function and predict protein-protein interactions.Bioinformatics identifi
高建群,1953年生,陕西临潼人。当代著名作家,陕西省文联副主席、作协副主席,享受政府特殊津贴有突出贡献专家,国务院跨世纪三五人才。其代表作《遥远的白房子》、《最后一个
目的:研究老年患者术后发生ICU谵妄的原因分析及护理方法、护理效果.方法:选择我院2019年06月-2020年06月期间收治的60例老年患者术后发生ICU谵妄的患者作为本次研究对象,采
The completion of the human genome project directly benefits the rapid development of DNA microarray technology.The application of microarrays in the study