Poly γ-glutamic acid (γ-PGA) /ε-polylysine (ε-PL) hydrogel was prepared by carbodiimide (EDC) and N-hydroxysuccinimide (NHS) ,FT-IR proved the amide bond as crosslinking linkage.The influence of PGA/PL molar ratio and EDC/NHS concentration was studied,and the surface and cross-section morphology of hydrogels was visualized by SEM,results showed a regularly porous structure with 20 μm pore size in average.The gelation time was detected and confirmed to be rapid.A brief but effective optotype method was developed to quantitatively evaluated the optical transparency,results signified the excellent optical clarity of PGA/PL hydrogels.The swelling kinetics in DI water,normal saline and PBS solution was investigated as well.It is found that the swelling kinetic followed a Voigt model in DI water and a pseudo-second-order model in normal saline and PBS solution respectively.The variesswelling degree were attributed to swelling theory based on physical and chemical structure of hydrogels.The swelling behavior was also sensitive to pH and show intelligent to regulate pH within limit.The mechanicalproperty study indicated that compressive modulus of PGA/PL hydrogels were 8 KPa in average.On the whole,these results suggested that EDC/NHS cross-linked PGA/PL hydrogels have high potential to be used for tissue engineering and medical.