Ad.RGD-ING4对人鼻咽癌细胞放疗增敏的实验研究

来源 :中华医学会2014全国耳鼻咽喉头颈外科中青年学术会议 | 被引量 : 0次 | 上传用户：lw10251

【摘要】

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【作者】

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王奕鸿刘济生

【机构】

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苏州大学附属第一医院耳鼻咽喉科 215000

【出处】

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中华医学会2014全国耳鼻咽喉头颈外科中青年学术会议

【发表日期】

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2014年3期

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目的 1.RGD修饰的腺病毒介导的ING4 (Ad.RGDING4)对人鼻咽癌细胞CNE放射增敏效应.2.初步探讨RGD修饰的腺病毒介导的ING4 (Ad.RGD-ING4)对人鼻咽癌细胞放射增敏的作用机制.方法体外:用QBI-293A细胞扩增Ad.RGD-ING4并测定效价;检测Ad.RGD-ING4在CNE人鼻咽癌细胞中感染效率,确定最佳感染复数;按重组腺病毒的最佳感染复数将Ad.RGD-ING4体外感染CNE人鼻咽癌细胞,使用RT-PCR及Western blot方法检测ING4基因在CNE中的转录及表达;不同放射剂量照射CNE细胞,用An nexin-V-P E/7-AAD双染色的FCM检测细胞凋亡率以寻找合适的实验放射剂量;MTT法检测Ad.RGDING4联合放疗对CNE细胞的生长抑制作用;流式细胞术(FCM)检测Ad.RGD-ING4联合放疗对CNE细胞的周期生长的影响和促凋亡效应;运用RT-PCR检测Ad.RGD-ING4联合放疗对CNE细胞中Bax、Bcl-2、P21 mRNA的表达水平的影响并运用Western blot检测以分析Survivin、Caspase3凋亡相关蛋白表达的变化.

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Ad.RGD-ING4对人鼻咽癌细胞放疗增敏的实验研究

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