Vibrio parahaemolyticus is an important human pathogen which can cause gastroenteritis when consumed in raw or partially-cooked seafood.In this paper,27 suspected Vibrio parahaemolyticus strains were isolated from 60 seafood by using TCBS agar and CHROMager vibfio colored medium in preliminary screening,The 27 strains were all defined to be Vibrio parahaemolyticus via biochemistry tests in the aRer identification,among these strains 22 were isolated from fresh seafood and 5 wore isolated from frozen seafood.