Tang Luo Ning (TLN) is a Chinese prescription used traditionally as a medicine to treat diabetic peripheral neuropathy (DPN).In this study, we used streptozocin induced rats to examine the effects of TLN on oxidative stress of DPN.DPN is a common complication of diabeies.Prolonged hyperglycemia resulted in oxidative stress is the major mechanism of DPN.TLN was administered at a dose of 10.9 and 21.2 g/kg body weight/day for 12 weeks.TLN treatments for 15 weeks reversed the deficiency of motor nerve conduction (MNCV) and sensory nerve conduction (SNCV), as well as reduced the thresholds of thermal perception.This functional improvement of sciatic nerve was accompanied by amelioration of structural changes exemplified by a lesser demyelination and a milder axonal atrophy in TLN-treated DPN rats.The loss body weight and high blood glucose did not change significantly.TLN also reduced the Reactive Oxygen Species (ROS) levels and increased the Total antioxidative capacity (T-AOC).TLN attenuated oxidative stress was involved in decreasing the expression of Bax and cytochrome c (Cyto C) as well as increasing the expression of B-cell CLL/lymphoma 2 (Bcl-2), nuclear erythroid 2-related factor 2 (Nrf2), and γ-glutamylcysteine synthetase (γGCS).