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A Gram-staining-negative,orange-pigmented,non-motile,aerobic bacterial strain,designated GYP20T,was isolated froma culture of the alga Picochlorum sp.,a promising feedstock for biodiesel production,which was isolated from the India Ocean.Growth was observed at temperatures from 20 to 37℃,at salinities from 0 to 3%and at pH from 5 to 9.Mg2+ and Ca2+ ions were required for growth.Phylogenetic analysis based on 16S rRNA gene sequencing revealed that the strain was a member of the genus Phaeodactylibacter,which belongs to the family Saprospiraceae.Strain GYP20T was most closely related to Phaeodactylibacter xiamenensis KD52T(95.5%sequence similarity).The major fatty acids were iso-C15:1 G,iso-C15:0,iso-C17:0 3-OH and Summed Feature 3.The predominant respiratory quinone was menaquinone-7(MK-7).The polar lipids of strain GYP20T were found to consist of diphosphatidylglycerol,phosphatidylethanolamine,phosphatidylglycerol,four unidentified glycolipid,two unidentified phospholipid and three unidentified aminolipid.According to its morphology,physiology,fatty acid composition and 16S rRNA sequence data,the novel strain most appropriately belongs to the genus Phaeodactylibacter,but can readily be distinguished from known Phaeodactylibacter xiamenensis GYP20T.The name Phaeodactylibacter luteus sp.nov.is proposed for strain GYP20T(=MCCC 1F01222T =KCTC 42180T).