论文部分内容阅读
Objective To explore the key regulatory genes associated with lung cancer in order to reduce its occurrence and progress through silencing these key genes.Method:In order to explore the key regulatory genes involved in lung adenocarcinoma,we performed the combination of gene array and bioinformatics analysis to compare genes transcription profiles in 3 monoclonal cell strains with high,medium or low metastatic abilities,which are separated from SPC-A-1sci and SPC-A-1 cell lines by limiting dilution monoclone assay,we then analyzed those genes biological activities by knockdown their expression in SPC-A-1 sei cells using siRNA and lenti-virus shRNA vector respectively,and then measured those cells abilities of invasion and migration in vitro,and inducing occurrence and metastasis of lung adenocarcinoma in vivo.Results:3 monoclonal cell strains characterized with high,medium or low metastatic abilities are successfully selected.Gene array and bioinformatics analysis implied osteopontin,LAMB3,and INTB 1 are key genes involved in lung adenocarcinoma.Knockdown of these genes decrease the ability of migration and invasion in vitro,and suppresses murine lung adenocarcinoma growth and metastasis in vivo.Conclusion:We confirmed that osteopontin,LAMB3,INTB1 play important roles in regulating occurrence and metastasis of lung adenocarcinoma,which provide important clues to understand the molecular mechanism of metastasis and contribute to the therapeutical treatment of lung cancer.