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目的 为研究肥大细胞在大鼠输卵管急性沙眼衣原体 (Chlamydialtrachomatis,CT)感染中的作用。方法 选择成年雌性SD大鼠 6 0只 ,通过手术从一侧卵巢囊接种沙眼衣原体D型株 ,对照组接种 2 -SPA缓冲液。分别于感染后第 1/ 2d、第 7d、第 14d将大鼠处死 ,取手术侧的输卵管常规固定、脱水、包埋。结果 S -P法显示 :输卵管局部的CD4 + T细胞和血管内皮细胞粘附分子 (VCAM - 1)的表达均较对照组明显增强 (P <0 0 1)。改良的甲苯胺蓝染色法显示 :感染组肥大细胞较对照组数量有显著性增高 (P <0 0 5 ) ,并且其变化趋势与CD4 + T细胞和VCAM - 1表达的变化趋势一致。结论 可以推测 ,沙眼衣原体感染引起急性输卵管炎时 ,肥大细胞通过促进炎症局部小静脉内皮细胞上VCAM -1的表达 ,诱导CD4 + T细胞的浸润 ;然后分泌IL - 4等细胞因子促进CD4 + T细胞向TH2 细胞方向转化 ,不利于机体清除沙眼衣原体 ,从而使发生局部输卵管病理损伤的可能性增加。
Objective To investigate the role of mast cells in the pathogenesis of tubal acute pancreatic trachoma (Chlamydial trachomatis, CT) in rats. Methods Sixty adult female Sprague-Dawley rats were selected. One of the ovarian cysts was inoculated with D type of C. trachomatis by surgery, and the control group was inoculated with 2-SPA buffer. Rats were sacrificed on day 1 / 2d, day 7d, and day 14d after infection, and the oviduct on the operative side was routinely fixed, dehydrated and embedded. Results The S-P method showed that the expression of CD4 + T cells and vascular endothelial cell adhesion molecule (VCAM - 1) in the fallopian tube was significantly higher than that in the control group (P <0.01). The modified toluidine blue staining showed that the number of mast cells in the infected group was significantly higher than that in the control group (P <0.05), and the trend was the same as that of CD4 + T cells and VCAM - 1 expression. Conclusions It can be inferred that when C. trachomatis infection causes acute salpingitis, mast cells induce the infiltration of CD4 + T cells by promoting the expression of VCAM - 1 on inflammatory small venule endothelial cells, and then secrete IL - 4 and other cytokines to promote CD4 + T Cells into the direction of TH2 cells, is not conducive to the body to clear Chlamydia trachomatis, so that the occurrence of local tubal pathological damage increased possibility.