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目的观察中药复方肠露灌肠剂对小鼠免疫性溃疡性结肠炎(ulcerative colitis,UC)模型的治疗作用。方法将实验小鼠随机分为正常组、模型组、肠露高剂量组(高剂组)、肠露低剂量组(低剂组)、柳氮磺吡啶(SASP)组。除正常组外,其余小鼠用兔结肠黏膜蛋白免疫法制作实验UC模型。给药治疗21天后,观察各组小鼠的疾病活动指数、体重变化,然后处死小鼠,取全段结肠,称结肠湿重,测肠重指数,比较各组小鼠的疾病活动指数、体重变化、结肠炎症反应及结肠组织病理学改变。结果肠露高、低剂量组,SASP组疾病活动指数明显减少,体重改善,结肠炎症反应减轻,与模型组比较差异有显著性(P<0．05或P<0．01);病理检查示:模型组小鼠结肠黏膜糜烂及溃疡形成、炎细胞浸润明显,肠壁充血伴出血,水肿明显;SASP组小鼠结肠黏膜未见明显糜烂及溃疡形成,但炎细胞浸润较明显,肠壁轻度充血,轻度水肿;肠露高刺组小鼠结肠黏膜无糜烂及溃疡形成,部分炎细胞浸润,肠壁轻度充血、无水肿。结论中药复方肠露灌肠剂对小鼠免疫性UC有明显的疗效。
OBJECTIVE: To observe the therapeutic effect of Chinese compound compound bowen decoction on immune ulcerative colitis (UC) model in mice. Methods The mice were randomly divided into normal group, model group, high dose group (high dose group), low dose group (low dose group), and sulfasalazine (SASP) group. Except the normal group, the other mice were immunized with rabbit colon mucosa to make experimental UC models. After 21 days of drug treatment, the disease activity index and body weight of each group of mice were observed. The mice were then sacrificed. The whole colon was taken, the wet weight of the colon was weighed, and the intestinal weight index was measured. The disease activity index and body weight of each group of mice were compared. Changes, colonic inflammatory response, and histopathological changes in the colon. Results The high-dose and low-dose bowel group showed significantly reduced disease activity index, improved body weight, and reduced colonic inflammatory response in the SASP group. There was a significant difference compared with the model group (P<0.05 or P<0.01). : In the model group, mucous membrane erosion and ulceration of colon, inflammatory cell infiltration, intestinal hemorrhage with hemorrhage and edema were evident; in the SASP group, there was no obvious erosion and ulceration in colon mucosa, but inflammatory cell infiltration was more obvious and the intestinal wall was lighter. Degree of congestion, mild edema; intestinal hyperplasia group mice in the colon mucosa without erosion and ulceration, some inflammatory cell infiltration, mild intestinal congestion, no edema. Conclusion The Chinese herbal compound bowel enema has obvious curative effect on immune UC in mice.