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In order to study the influence of combined use of isoniazid and rifampicin to the activities of CYP1A2 and 3A4 of primary hepatocyte in healthy adult.we isolated health adult huaman primary hepatocytes from healthy adult human liver,and cultured for three days,then added isoniazid,rifampicin or both of them with different concentration in the range of maximum clinical blood concentration respectively.After cultured for another two days,substrate were added (phenacetin for CYP1A2,testosterone for CYP3A4) ,then measured peak area (unit: mAU.rnin) of their metabolite with high performance liquid chromatography to assess the activities of CYP1A2 and 3A4.