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The purpose of this investigation was aimed at exploring whether ponatinib (AP24534), a novel effective TKI against T315I Bcr-Abl was active against D816V KIT.First, Western blotting analysis was applied to testify the effect of ponatinib on the signaling transduction involved in apoptosis, KIT pathway, and β-catenin signaling.Then, the status of apoptosis was analyzed by Annexin V/PI double staining with a FACScalibur flow cytometer.