论文部分内容阅读
The purpose of this study was to investigate whether autologous transplantation of adipose-derived mesenchymal stem cells(ADMSCs)has a neuroprotective effect against cerebral ischemia and reperfusion(I/R)injury in rats and to explore the possible underlying mechanisms.Adult male Sprague-Dawley rats were randomly assigned into the sham,I/R injury model(I/R),and model plus autologous transplantation of ADMSCs(ADMSC)groups.Cerebral I/R injury was induced by 2 h middle cerebral artery occlusion(MCAO)followed by reperfusion for 24 h.Rats in the I/R and ADMSC groups were intravenously injected with ADMSCs(2.0×106)and culture medium,respectively,at the onset of reperfusion and 12 h after reperfusion.Cerebral infarct volume was detected by triphenyltetrazolium chloride(TTC)staining.The histopathological changes and neuronal apoptosis in the ischemic penumbra were evaluated with H&E staining and the TUNEL assay,respectively.The nitric oxide(NO)content,caspase-3 activity,and the Bax/Bcl-2 protein ratio were also measured.