Objective: There are many clinical applications using cell-based products under the Medical Practitioners Act and the Medical Care Act in Japan,and almost of them are autologous cell therapies.A raw material has an individual specific activity depend on the condition of donor patient.If the efficacy of a treatment modality was evaluated,the conditional differences containing in the products would be one of important issues.Therefore,we evaluated the influences due to raw materials in the cell expansions of peripheral blood mononuclear cells(PBMCs),which were accumulated by manufacturing for immunotherapy at the Biotherapy Institute of Japan(BIJ).In this study,the influence of the storage period containing transit time after the collection of blood as a raw material was focused.Methods: PBMCs as a raw material was isolated from 50 mL of peripheral blood,and the expansion using a natural killer cell expansion kit(BINKIT(R),BIJ,Japan)was carried out.The peripheral blood collections were carried out at Tokyo or Fukushima in Japan.PBMC isolation was started in same day when the blood was collected at Tokyo,and the isolation was treated the next day(after 24 hours)when collected at Fukushima.All the culture periods extracted was 20±2 days.Results: Recent 37 cases were picked up in a preliminary survey.The average number of cells collected was 3.6 x 109 when collected at Tokyo,and was 3.0 x 109 when collected at Fukushima.Both the growth rates of cells were close.