【摘 要】
：
α-Ketoglutarate(α-KG)is a important intermediate in tricarboxylic acid cycle,and is one of the major precursors for the synthesis of most biochemical subs
【机 构】
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CollegeofBiotechnologyandPharmaceuticalEngineering,NanjingTechUniversity,No.5XinmofanRoad,Nanjing210
【出 处】
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2016年分析化学前沿国际研讨会及中美分析化学研讨会
论文部分内容阅读
α-Ketoglutarate(α-KG)is a important intermediate in tricarboxylic acid cycle,and is one of the major precursors for the synthesis of most biochemical substances.Here we report on a rapid and highly sensitive amperometric biosensor for the detection of α-KG based on a glassy carbon electrode(GCE).The biosensor was constructed by three steps.Firstly,Au nanoparticle(Aunano)/reduced graphene oxide(rGO)composite film was one-step electrodeposited onto the bare GCE.Then the glutamate dehydrogenase(GLUD)was droped into the Aunano/rGO-GCE.Further,the GLUD/Aunano/rGO-GCE was dipped in 2%glutaraldehyde for 3s to form a protective coating.Scanning electron microscopy shed useful insights into the morphology of the modified electrode.The Aunano/rGO-GCE demonstrated excellent direct electrocatalytic oxidation toward β-nicotinamide adenine dinucleotide(NADH),with a negative shift of~400 and~310 mV,in comparison to the bare electrode and Aunano–GCE,respectively.The GLUD/Aunano/rGO-GCE leaded to effective amperometric detection of α-KG through monitoring of the NADH consumption.The biosensor developed in this study displayed a high sensitivity of 2.97 μA μM-1,a linear range of from 43.03 to 234.25 μM with a limit of detection of 10 μM and the response was fast(less than 6s).This proof of concept research indicates that the GLUD/Aunano/rGO-GCE biosensor holds great promise for real-time electrochemical measurements of α-KG.
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