【摘 要】
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Acid-sensing ion channels (ASICs) are believed to be homo-or heteromeric complexes,which have been verified by classical methods such as co-immunoprecipitat
【机 构】
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Department of Neurobiology, Institute for Neuroscience, Zhejiang University School of Medicine, Hang
【出 处】
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中国神经科学学会第四次会员代表大会暨第七届全国学术会议(The 7th Biennial Meeting and the
论文部分内容阅读
Acid-sensing ion channels (ASICs) are believed to be homo-or heteromeric complexes,which have been verified by classical methods such as co-immunoprecipitation or electrophysiological assays.However,the exact subunit combinations of ASICs in living cells have not been established yet.Here,we apply assays based on fluorescence resonance energy transfer (FRET) between GFP color mutants CFP and YFP to investigate ASIC assembly directly in living cells.Homomerization as well as heteromerization of different combinations ofASIC subunits were found.In addition,our results suggest the formation of heteromeric 1 a/2a channels of stoichiometry consisting of at least two 1 a subunits and two 2a subunits.Similar stoichiometry was observed from heteromeric 1a/2b and 2a/2b channels.Our results imply that these heteromeric ASIC channels contain at least four subunits.
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