Rutin is a kind of natural flavonoid from plants.It is applied widely in the food,pharmaceutical,and cosmetic industries for its peculiar activities such as antioxidation[1,2],anti-inflammation,anti-hypertension[3] and The aim of this work was to give some references to industrial production,quality assurance and supervision of the plant after developing a simple,sensitive,selective and reliable method for the determination of rutin in Fructus Sophorae.