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The chiral recognition ability of chiral stationary phases(CSPs) is the key point of chiralseparation technique by high-performance liquid chromatography(HPLC).1 Stereoregular helicalpoly(phenylacetylene) derivatives bearing chiral side chains have been proved as CSPs for HPLCseparation of enantiomers.2 However,the influences of the chiral groups and their chiralrecognition mechanism as CSPs for HPLC are still not well-known and are attractive researchthemes till now.