【摘 要】
：
目的 帕金森病(Parkinson Disease,PD)发病机制目前尚不明确,近年来T细胞在PD发病中的作用受到重视：研究发现PD患者及动物模型中存在T细胞亚群的改变,提示PD中存在T细胞免疫的紊乱；而且在PD的黑质病变区存在T细胞的浸润,使用地塞米松后,T细胞的浸润减轻,PD的损伤程度也减轻.T细胞表面抗原CD4及CD8,作为特异性细胞免疫的重要分子,参与抗原提呈和细胞特异性杀伤作用.本研究中
【机 构】
：
第三军医大学神经生物教研室,重庆400038 成都医学院神经生物教研室,成都610081
【出 处】
：
中国神经科学学会第四次会员代表大会暨第七届全国学术会议(The 7th Biennial Meeting and the
论文部分内容阅读
目的 帕金森病(Parkinson Disease,PD)发病机制目前尚不明确,近年来T细胞在PD发病中的作用受到重视：研究发现PD患者及动物模型中存在T细胞亚群的改变,提示PD中存在T细胞免疫的紊乱；而且在PD的黑质病变区存在T细胞的浸润,使用地塞米松后,T细胞的浸润减轻,PD的损伤程度也减轻.T细胞表面抗原CD4及CD8,作为特异性细胞免疫的重要分子,参与抗原提呈和细胞特异性杀伤作用.本研究中利用分别敲除CD4+T细胞和CD8+T细胞的敲基因动物,在MPTP致C57BL/6小鼠的PD模型中,观察基因敲除后DA能神经元的损伤程度及胶质细胞活化情况,以阐明CD4+T细胞和CD8+T细胞在PD发病中的作用,为细胞免疫在PD中的作用提供实验基础.方法 雄性C57BL/6小鼠,体重25 9左右.野生型(wild-type,WT)、CD4敲除型(CD4 knock-out,CD4-KO)、CD8敲除型(CD8 knock-out,CD8-KO)；同时设立生理盐水对照组、MPTP模型组.MPTP购自Sigma公司,腹腔注射30 mg/kg每天,共5天.造模后10d,取双侧纹状体及黑质.
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