肠杆菌(Enterobacter sp.CN1)甲酸代谢及相关产氢的研究

来源 :第十六次全国环境微生物学学术研讨会 | 被引量 : 0次 | 上传用户：lyling0411

【摘要】

：

【作者】

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邱玉锋黄江伦镜盛胡忠

【机构】

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汕头大学生物系,汕头,515063

【出处】

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第十六次全国环境微生物学学术研讨会

【发表日期】

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2013年10期

【关键词】

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甲酸代谢途径 NiFe-氢酶克隆重组表达

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　　目的探讨肠杆菌(Enterobacter sp.CN1)木糖产氢代谢中的甲酸盐途径以及研究相关产氢基因提高产氢量.方法以木糖高效产氢菌Enterobacter.sp CN1为研究对象,通过其甲酸代谢产氢、甲酸对木糖产氢的影响；以及甲酸盐代谢途径中甲酸氢裂解酶激活子基因(fhlA)和NiFe-氢酶基因(hycE, hycG)的克隆、表达以及重组菌株的产氢分析.结果甲酸为唯一碳源进行发酵产氢,甲酸几乎完全转化为氢气,但高浓度的甲酸有轻微的抑菌作用；甲酸能促进CN1利用木糖产氢；重组菌株的发酵产氢实验表明,与野生菌相比,FHLA蛋白和NiFe-氢酶过量表达的重组菌单位菌体产氢量分别提高了18.8％和23％.

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肠杆菌(Enterobacter sp.CN1)甲酸代谢及相关产氢的研究

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