【摘 要】
：
Telomere is the end of a chromosome, which is a specialized structure involved in the replication and stability of the chromosome. Quantitative real-time polymerase chain reactions (qRT-PCR) have beco
【机 构】
：
Biotechnology Institute, Dalian Jiaotong University, Dalian 116028, China
【出 处】
：
2011年中国药学大会暨第11届中国药师周
论文部分内容阅读
Telomere is the end of a chromosome, which is a specialized structure involved in the replication and stability of the chromosome. Quantitative real-time polymerase chain reactions (qRT-PCR) have become the method of choice for rapid, sensitive, quantitative comparison of telomere length[1,2]. Cawthon[1] designed oligonucleotide primers tel1 and tel2 that hybridize to the TTAGGG and CCCTAA repeats and used AmpliTaq Gold DNA polymerase on the Applied Biosystems Prism 7700 sequence detection system. Gil and Coetzer[2] modified Cawthons method and described the parameters and reagents required to measure telomere length using the Roche LightCycler. However, the advantage of the primers tel1b and tel 2b was not mentioned. In recent years, β-globin is used more often than 36B4 as the reference single-copy gene[e.g., 3-5], but the reason of giving up 36B4 was not put forward. Moreover, the real-time thermal cycler used in our laboratory, as well as in numerous laboratories worldwide, is the Takara PCR Thermal Cycler Dice Real Time System (TP800) using the modified Takara Ex Taq HS. In the beginning we attempted the assay by applying the conditions published for the Prism 7700 and LightCycler onto the TP800. However, owing to different reagents and inherent differences between these instruments, substantial optimization was required for the technique to be applied to the TP800. Here we describe the reagents and conditions required to measure telomere length using the TP800.
其他文献
目的：对茜草科植物华钩藤[ Uncaria sinensis（Oliv.）Havil.]的干燥带钩茎枝的木脂素类化学成分进行研究。 方法：用95％乙醇对华钩藤的干燥带钩茎枝进行提取，分别用石油醚、乙酸乙酯、氯仿和正丁醇萃取，对正丁醇部位采用各种柱色谱进行分离，通过NMR、 MS 和CD 等波谱学数据分析进行结构鉴定。 结果：分离鉴定了4 个木脂素类化合物的结构：（2R，3R，4S）-lyoni
经考证《植物名实图考》中的星宿菜、珍珠菜、落地梅、过路黄、红丝毛根、刘海节菊、临时救、扯根菜，黄莲花分别为泽珍珠菜、狭叶珍珠菜小叶珍珠菜（重楼排草）、过路黄、星宿菜、福建过路黄、裸头过路黄、狼尾草（重穗排草）、黄莲花。
目的：观察和比较红丝线草醇提取物和酸提取物对肾性高血压大鼠的血压的影响，以及对血清 NO 和NOS 的影响。 方法：结扎SD 大鼠左肾动脉形成肾性高血压模型，手术成功后随机分为9 组，分别为红丝线草醇提物和酸水提取物高、中、低剂量组，卡托普利组，模型组，假手术组。灌胃给药3 周。于手术前、手术后第3 周末、给药后第1、2、3 周末测定血压；给药后第3 周末采用经颈动脉插管采血，测血清NO、NOS
目的：首次对苦瓜(Momordica Charantia L.)进行酸水解，对其水解产物进行分离。 方法：通过硅胶柱色谱、Sephadex LH-20 柱色谱和HPLC等多种色谱手段。 结果：分离得到5 个化合物，分别为 (19R,23E)-5β,19-epoxy-19-methoxycucurbita-6,23,25-trien-3β-ol（化合物Ⅰ）、β-谷甾醇（化合物Ⅱ）、苦瓜二醇A（化
Develop a HPLC-MS/MS method for the quantification of platycodin D (PD) in rat plasma and apply it to gain the main pharmacokinetic parameters of PD after oral administration of PD alone and Platycodi
目的：了解疝气手术抗菌药物使用情况，为临床合理使用抗菌药物提供依据。 方法：采用回顾性调查的方法对102 例疝气手术患者抗菌药物应用进行统计分析。 结果：102 例疝气手术的患者中90 例使用了抗菌药物，使用率为88.2％，无指征用药的比率为20.6%，其中一联用药占85.6%，二联用药占14.4%。预防用药共13 类，使用频次较高的品种为头孢西丁（23.1%）、氟氯西林（11.1%）、头孢替
Tetramethylpyrazine (TMP) is one of the most important active ingredients of a Chinese herb Ligusticum wallichii Franchat, which is widely used for the treatment of cardiovascular diseases. Several fa
目的：建立同时测定复方盐酸奈福泮胶囊中盐酸奈福泮和萘普生含量的高效液相色谱方法。 方法：采用色谱柱为Apollo C18柱（250 mm×4.6 mm, 5μm），以庚烷磺酸钠溶液（取庚烷磺酸钠2.02 g，加水900 mL使溶解，加三乙胺2 mL，用稀磷酸调节pH3.0，加水至1000 mL）-乙腈（53：47）为流动相，流速为1.0mL·min-1，检测波长为215 nm。 结果：盐酸奈福
Cytochrome P450 2D6 (CYP2D6), a member of the cytochrome P450 mixed-function oxidase system, is one of the most important enzymes involved in the metabolism of xenobiotics in the body. Also, many subs
The phase II metabolizing enzyme UDP-glucuronosyltransferase (UGT) is a glycosyltransferase (EC 2.4.1.17) that catalyzes the glucuronidation reaction (King et al. 2000). The UGT enzyme is responsible