【摘 要】
：
目的:体外建立心肌微血管内皮细胞(cardiac microvascular endothelial cells,CMVEC)的实验模型,探讨干扰Notch信号通道对心肌微血管内皮细胞间质转分化的作用.方法:采用体外酶消化法分离培养大鼠CMVEC,通过两次差速贴壁纯化细胞.应用倒置相差显微镜观察细胞形态,免疫荧光法检测细胞表面特异性抗原.构建Notch1RNAi慢病毒.实验分为三组:A:正常对照组
【出 处】
：
第二十四届长城国际心脏病学会议、亚太心脏大会暨国际心血管病预防与康复会议(The 24th Great Wall Int
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目的:体外建立心肌微血管内皮细胞(cardiac microvascular endothelial cells,CMVEC)的实验模型,探讨干扰Notch信号通道对心肌微血管内皮细胞间质转分化的作用.方法:采用体外酶消化法分离培养大鼠CMVEC,通过两次差速贴壁纯化细胞.应用倒置相差显微镜观察细胞形态,免疫荧光法检测细胞表面特异性抗原.构建Notch1RNAi慢病毒.实验分为三组:A:正常对照组;B:低氧培养组;C:低氧+Notch1RNAi组.通过免疫荧光双染法检测CD31和FSP-1的表达,使用RT-qPCR检测Notch1下游基因HES-1的表达以及FSP-1、α-SMA的表达.
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