【摘 要】
：
原子力显微镜技术(AFM)的出现使得对生物大分子的单分子观测及操纵成为可能[1,2].AFM 友好的工作环境(比如,可以在溶液中工作)使得无论是空气中或者溶液中的高精度成像都
【出 处】
：
中国化学会生物物理化学专业委员会第四届全国生物物理化学会议
论文部分内容阅读
原子力显微镜技术(AFM)的出现使得对生物大分子的单分子观测及操纵成为可能[1,2].AFM 友好的工作环境(比如,可以在溶液中工作)使得无论是空气中或者溶液中的高精度成像都可以实现.基于原子力显微镜技术的单分子力学谱(以下简称单分子力谱)的出现使得人们能够对单个大分子进行力学操纵和加工,从而对分子结构与构象变化,分子间的相互作用以及反应历程实现单分子水平的实时-原位观测.通过传统的AFM 成像,可以实现对分子间作用模式(或者结合过程)的直观检测,而利用AFM 力谱方法可以实现对分子间(或分子内)作用强度的定量检测.
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