【摘 要】
：
Cell-based in vitro assays are a key tool for the assessment of compound-induced toxicity in early drug development before entering regulatory animal testin
【机 构】
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Group Head Mechanistic Toxicology Non-Clinical Safety,Pharma Research Hoffmann-La Roche AG Switzeral
【出 处】
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中国上海第七届国际新药发明科技年会
论文部分内容阅读
Cell-based in vitro assays are a key tool for the assessment of compound-induced toxicity in early drug development before entering regulatory animal testing.A non-invasive and label-free tool,which allows continuous monitoring of cellular behavior is provided by the xCELLigence Real-Time Cell Analyzer (RTCA) System,co-developed by Roche and ACEA Biosciences.This system is based on measuring electric impedance generated by adherent cells,which interact with a microelectrode biosensor at the bottom of the well of a cell culture plate.The readout,called "cell index" (CI),measures morphological alterations of cells caused by e.g.drug-treatment.The technique allows hands-free dynamic monitoring of cellular events,such as proliferation,cell death,adhesion,spreading and shrinking in a 96-well format over an extended period of time.
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