【摘 要】
：
To produce ethanol from lignocellulosic materials is considered as an economically viable energy strategy.However, at least three bottlenecks need to be ove
【机 构】
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State Key Laboratory of Microbial Technology in Shandong University,China
【出 处】
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The 6th World Bioenergy Symposium(WBS 2012)(第六届国际生物能源会议)
论文部分内容阅读
To produce ethanol from lignocellulosic materials is considered as an economically viable energy strategy.However, at least three bottlenecks need to be overcome, which are the co-utilizing of xylose with glucose in the substrate, the expensive cost of cellulase, and the toxic of lignocellulose hydrolyzate.Here, we constructed the high-efficiency xylose fermentation strains using genetic engineering and adaptive evolution strategy.The transcription work had done and guided the further modification.Aimed to reduce the cellulase cost, the protein secretion pathway and N-glycosylation pathway were modified for CBP (Consolidated Bioprocessing) yeast construction.Furthermore, the resistant of yeast to the inhibitors in lignocellulose hydrolyzate was evaluated and intensified.
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