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目的:研究清开灵注射液对局灶性脑缺血再灌注损伤的治疗时间窗。方法:线栓法制备昆明小鼠局灶性脑缺血再灌注损伤模型,分别在缺血后0、1、3、4、6h经尾静脉给予清开灵注射液,24h后进行神经功能评分,处死后取脑,TTC染色并计算脑梗塞体积。结果:各给药组均能显著减少梗塞体积,各组局灶功能损伤均有显著改善,一般功能损伤和Bederson评分只有0h给药组有显著改善。结论:清开灵注射液可减少局灶性脑缺血再灌注损伤小鼠的脑梗塞体积并改善其神经功能,其治疗时间窗可达到6h。
Objective: To study the therapeutic window of Qingkailing injection on focal cerebral ischemia-reperfusion injury. Methods: The model of focal cerebral ischemia-reperfusion injury in Kunming mice was established by suture method. Qingkailing injection was given via tail vein at 0, 1, 3, 4 and 6 h after ischemia, and neurological function scores , Sacrificed after brain, TTC staining and calculate the volume of cerebral infarction. Results: Each administration group could significantly reduce the infarct volume. The functional impairment of each group was significantly improved. The general functional impairment and Bederson score were significantly improved only in 0h group. Conclusion: Qingkailing injection can reduce cerebral infarction volume and improve neurological function in focal cerebral ischemia-reperfusion injury mice, and its therapeutic time window can reach 6h.