While 16S ribosomal RNAsequence-based identification of Nocardia species has become the gold standard,it is not without its limitations.We evaluated a novel approach encompassing the amplification of the Nocardia 16S-23S rRNA intergenic spacer(IGS) region followed by fragment analysis by capillary gel electrophoresis (CGE) of the amplified product for species identification of Nocardia.One hundred and forty-five Nocardia isolates (19 species) and four non-nocardiae aerobic actinomycetes were studied.