扇贝养殖水域褐潮期间一株优势微藻的分类地位研究

来源 :第四届全国藻类多样性和藻类分类研讨会 | 被引量 : 0次 | 上传用户:alei1001
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  近年来,我国河北秦皇岛扇贝养殖水域发生的大规模褐潮使我国成为世界上第三个受褐潮影响的国家,当地扇贝养殖产业受到严重危害.在对褐潮期间扇贝养殖水域浮游植物种群结构研究过程中,发现除褐潮藻抑食金球藻Aureococcus anophagefferens Hargraves etSieburth 外,还有一株微型藻密度很高,占优势地位.采用稀释法分离纯化到这一藻株,经透射及扫描电子显微镜观察,该藻株和硅藻门的多形微眼藻Minutocellus polymorphus(Hargr.& Guill.)Halse 极为相似,但形态上仍有部分差异.PCR 扩增其18S rDNA 序列,测序并构建系统进化树分析,结果显示与多形微眼藻Minutocellus polymorphus 的18S rDNA 部分序列相似度高达99%,但由于18S rDNA 的高度保守性,其分类阶元只能划分到属.结合形态学和分子生物学证据,推测这一优势微藻为微眼藻属Minutocellus Halse 的一新种,也可能是多形微眼藻Minutocellus polymorphus 的一个变种.本项目受海洋公益性行业科研专项(201205031-03)资助.
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