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Objectives: Recent studies suggest that the imbalance of the antioxidant status and abnormal immune function are involved in breast cancer; therefore, we investigate whether the immune and antioxidant statuses could be improved by the addition of antioxidant additives, such as whey protein concentrate (WPC).Methods: We analyzed the lymphocyte subpopulations, apoptotic markers, and mitochondria membrane potential by a flow cytometer and the generation of superoxide radical (O2-·) by ultra-chemiluminescence to investigate the effects of WPC on the immune function in the peripheral blood mononuelear cells (PBMCs) of the 25 breast cancer patients and 25 healthy controls.Additionally, the levels of reduced form glutathione (GSH) and oxidized form glutathione (GSSG) in the erythrocytes were analyzed by a capillary electrophoretic analyzer.Results: The GSH levels and GSH/GSSG ratios, and the numbers of CD3+ cells, CD4+ cells, and total lymphocytes in the patients were significantly lower than those in the controls (p<0.05 by Student t-test).Conversely, the numbers of CD56+ cells and the generation of O2-· in the patients were significantly higher than those in the controls (p<0.05 by Student t-test).After the supplementation with WPC, the numbers of total lymphocytes were significantly higher than those without the supplementation with WPC in the controls (p<0.05 by Student t-test); however, they were not significantly different in the patients.Conclusions: These results suggested that the immunological functions might be enhanced by the supplementation with WPC; however, the effects of WPC on the antioxidant statuses were not apparent.