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Highly porous ultrafine electrospun scaffolds, gelatin/PLLA and chitosan/PLLA were prepared by blending gelatin and PLLA, chitosan and PLLA respectively.This bilaminar texture is suitable for preparing a bilayer skin substitute.The characterization of gelatin/PLLA and chitosan/PLLA scaffolds were examined by scanning electron microscopy (SEM) and compared respectively.The biocompatibilities of the gelatin/PLLA and chitosan/PLLA scaffolds were assessed by attachment, proliferation and viability of cells on them.The human fibroblasts cell line WI-38 was inoculated on gelatin/PLLA and chitosan/PLLA scaffolds, pure PLLA scaffolds as control.Our study indicated that over 30% WI-38 cells could attach to the gelatin/PLLA and chitosar/PLLA scaffolds at 2h after seeding, while the attachment of the cells was only 15% on PLLA scaffolds.Both gelatin/PLLA and chitosan/PLLA scaffolds also exhibited a very good ability for proliferation of WI-38 cells.Cell growth on the gelatin/PLLA and chitosan/PLLA scaffolds showed dramatic improvement, indicating a much better biocompatibility in the blends contributed by gelatin and chitosan.MTT assay also demonstrated gelatin/PLLA showed better ability to enhance the growth and functions of the cells.These assays suggest that the electrospun gelatin/PLLA and chitosan/PLLA scaffolds are promising biomaterials with great biocompatibility for the development of skin tissue engineering.