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<正>Centella asiatica(L.) Urban is a widely distributed herbaceous plant with great medicinal value that has been extensively used in traditional Chinese medicine.For the effective quality control of C.asiatica,a feasible approach and control system is necessary.In this paper,a chemical fingerprint method(high-performance liquid chromatography,HPLC) was developed for investigating and demonstrating the variance of chemical components among different populations of C. asiatica,from 14 locations in China.The HPLC data showed considerable variation of chemical constituents among the C. asiatica populations.Four chemotypes was visually developed from the chromatographic profiles.The hierarchical clustering analysis further suggested that the samples were divided into four major groups.The grouping of the 14 C.asiatica populations in hierarchical clustering analysis was in good agreement with the visual comparison of their chromatograms,as demonstrated by the chemotype.In addition,the bioactive compound asiaticoside in this herb was quantitatively determined by a validated reverse-phase HPLC analysis.Chemotype I was identified as asiaticoside-rich chemotype and exhibited significantly higher amounts of asiaticoside.These finding and results provides a solid basis to establish good agriculture practices and select geo-authentic crude drug for C.asiatica.Meanwhile,both relative retention times and relative peak areas of characteristic compounds in chromatograms were calculated for identification and quantitative expression of the HPLC fingerprints.In method validation,relative standard deviation(RSD) of relative retention times and relative peak areas were of not more than 3%.It was concluded that the established method was considered to be suitable for fingerprint analysis to control the quality of C.asiatica.