Characterization of Virus-derived siRNAs and Their Host Targets from RBSDV-infected Japonica and Ind

来源 :中国植物病理学会2014年学术年会 | 被引量 : 0次 | 上传用户:miracle_l
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  RNA silencing plays important roles in defense against invading virus in plants.Virus-derived small interfering RNAs (vsiRNAs) can inactivate the viral genome and may target host mRNAs.Rice black-streaked dwarf virus (RBSDV) is transmitted to plant species by the small brown planthopper (SBPH), which severely affects rice and maize production in East Asia in recent years.VsiRNAs produced from their insect vectors are well described but those from their hosts are less characterized.Through deep sequencing, we examined vsiRNAs from infected leaves and shoots of RBSDV-infected two rice genotypes—japonica and indica.To our data, vsiRNAs identified in both genotypes were distributed along the whole genome of RBSDV.More vsiRNAs were derived from the positive strands and mostly were 21 ~ 22nt long.
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