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Grass carp reovirus (GCRV), the representative member of aquareovirus-C, serves as a model in studying the pathogenesis of aquareovirus.This study sought to isolate and characterize the receptor protein used by GCRV to gain entry into Ctenopharyngodon idellus kidney cell (CIK).The 37-kDa laminin receptor precursor (LRP) was identified as the interacting partner for outer capsid protein VP5 of GCRV by using the yeast two-hybrid technology in Saccharomyces cerevisiae.