Chimeric-gene Hairpin Structures Affect Virus Resistance to RSV and RBSV Mediated by RNA Silencing i

来源 :中国植物病理学会2014年学术年会 | 被引量 : 0次 | 上传用户:icnew
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Two types of hairpin RNA (hpRNA) constructions were designed using a chimeric gene formed from two genes, the coat protein (CP) gene or the silencing suppressor gene, from the Rice stripe virus (RSV) and Rice black-streaked dwarf virus (RBSDV), respectively;one type generated a single hairpin structure, whereas the other formed a two-hairpin structure.Two constructs, pRVDCPSH (double CP gene fragments, single hairpin), pRVDCPDH (double CP gene fragments, double hairpins) were individually introduced into rice plants.
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