Preselection of High Specificity CRISPR/Cas9 Target LocusAssociated withBovine Mammary Gland Bioreac

来源 :第九次全国动物生物技术学术研讨会 | 被引量 : 0次 | 上传用户：glamour269

【摘要】

：

【作者】

：

Tong Qi Yong Zhang

【机构】

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College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi, China

【出处】

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第九次全国动物生物技术学术研讨会

【发表日期】

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2015年1期

【关键词】

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mammary gland bioreactor CRISPR/Cas9 bovine double-strand breaks(DSB) homologous

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　　Type Ⅱ CRISPR (clustered, regularly interspaced, palindromic repeats)/Cas9 (CRISPR-associated endonuclease 9) system has been exploited for locus-specificgenome editing in a wide variety of model organisms and generated tremendous research upsurge due to certain characteristics-simplicity, efficiency and multiplex targeting functionalities.However, various assessments have shown thatunwanted mutations associated with off-target DNA cleavages could be the restriction element in certain applications.

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Preselection of High Specificity CRISPR/Cas9 Target LocusAssociated withBovine Mammary Gland Bioreac

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