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Type Ⅱ CRISPR (clustered, regularly interspaced, palindromic repeats)/Cas9 (CRISPR-associated endonuclease 9) system has been exploited for locus-specificgenome editing in a wide variety of model organisms and generated tremendous research upsurge due to certain characteristics-simplicity, efficiency and multiplex targeting functionalities.However, various assessments have shown thatunwanted mutations associated with off-target DNA cleavages could be the restriction element in certain applications.